Facultatea de Fizică
Universitatea "Al.I.Cuza" Iaşi
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| Titlu: Studiul transportului ionic prin biomembrane artificiale |
| Autori: Constantin Petrică Cristin, Roxana Andronache, Tudor Luchian |
| Afiliere: Facultatea de Fizica, Universitatea “Al.I.Cuza”,
Bv. Carol I, no. 11, Iasi 700506, Romania |
| Abstract: Reconstituted bilayer membranes were formed from two lipid monolayers at an airwater
interface by the apposition of their
hydrocarbon chains, when an aperture in a Teflon partition separating the two aqueous phases where the membrane is to be
formed, is lowered below the interface. Formation of the membrane is monitored by an increase of the electrical capacity of the
system, measured with the voltage clamp method. Electrical resistance of the unmodified membrane is analogous to that of
conventional natural lipid membrane lacking ion channels and other transporter proteins. However, incorporation of certain antimicrobial
peptides into the reconstituted membrane was seen to promote the formation of ionchannellike
‘defects’, which
greatly increase the overall conductance of the membrane. |
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